Tris(hydroxymethyl)aminomethane (TRIS)
Tris(hydroxymethyl)aminomethane (TRIS) is a widely used organic compound with applications in biochemistry, molecular biology, and pharmaceutical industries. Known for its buffering capacity, Tris(hydroxymethyl)aminomethane (TRIS) maintains stable pH levels in various laboratory and industrial processes.
Product Specifications
Physical and Chemical Properties
- Molecular Formula: C4H11NO3
- Molecular Weight: 121.14 g/mol
- Appearance: White crystalline powder
- Solubility: Highly soluble in water, slightly soluble in ethanol
- Melting Point: 167-172°C
- pH Range: 7.0-9.0 (for buffering applications)
Purity Grades
| Grade | Purity | Application |
|---|---|---|
| Reagent Grade | ≥99% | General laboratory use |
| Molecular Biology Grade | ≥99.5% | DNA/RNA electrophoresis |
| Pharmaceutical Grade | ≥99.9% | Drug formulation |
Applications of Tris(hydroxymethyl)aminomethane (TRIS)
- Buffer solution preparation (e.g., TAE, TBE)
- Protein electrophoresis and immunoblotting
- DNA/RNA extraction and purification
- Enzyme stabilization
- Cell culture media preparation
Packaging Options
| Package Size | Form | Shelf Life |
|---|---|---|
| 100g | Bottle | 3 years |
| 500g | Bottle | 3 years |
| 1kg | Bag | 2 years |
| 25kg | Drum | 2 years |
Safety Information
- Wear appropriate personal protective equipment (PPE)
- Avoid inhalation of dust
- Store in a cool, dry place away from acids
- In case of eye contact, rinse immediately with water
FAQ
What is Tris(hydroxymethyl)aminomethane (TRIS) used for?
Tris(hydroxymethyl)aminomethane (TRIS) is primarily used as a buffering agent in biological and biochemical applications. It helps maintain stable pH conditions in solutions, particularly in the pH range of 7.0-9.0. Common uses include electrophoresis, protein purification, and molecular biology techniques.
How should I store Tris(hydroxymethyl)aminomethane (TRIS)?
Tris(hydroxymethyl)aminomethane (TRIS) should be stored in a tightly sealed container at room temperature, away from moisture and acidic substances. Proper storage ensures long-term stability and prevents degradation of the compound.
What is the difference between Tris base and Tris HCl?
Tris base is the free base form of Tris(hydroxymethyl)aminomethane (TRIS), while Tris HCl is its hydrochloride salt. The main difference lies in their pH when dissolved in water. Tris base is alkaline, while Tris HCl is acidic. They are often used together to create buffer solutions with specific pH values.